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were collected from within St. Anns Bank Marine Protected Area. This MPA exists offshore of Cape Breton Island, Nova Scotia, Canada.","boundingBox":{"minLatitude":45.989473,"maxLatitude":46.280412,"minLongitude":-59.210587,"maxLongitude":-58.852367,"globalCoverage":false}}],"temporalCoverages":[{"@type":"range","start":"2022-08-14T00:00:00.000+00:00","end":"2022-08-16T00:00:00.000+00:00"}],"keywordCollections":[{"thesaurus":"GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml","keywords":["Occurrence"]}],"project":{"title":"Biodiversity Monitoring in St. Anns Bank Marine Protected Area using Environmental DNA - 2022","contacts":[{"type":"ADMINISTRATIVE_POINT_OF_CONTACT","primary":false,"userId":[],"firstName":"Nick","lastName":"Jeffery","position":[],"email":[],"phone":[],"homepage":[],"address":[]},{"type":"ADMINISTRATIVE_POINT_OF_CONTACT","primary":false,"userId":[],"firstName":"Ryan","lastName":"Stanley","position":[],"email":[],"phone":[],"homepage":[],"address":[]}],"funding":"This work was funded by the Marine Conservation Targets program at Fisheries and Oceans Canada.","awards":[],"studyAreaDescription":"The study area was within St. Anns Bank Marine Protected Area, located offshore of eastern Cape Breton, Nova Scotia, Canada.","relatedProjects":[]},"samplingDescription":{"studyExtent":"Samples were collected from within St. Anns Bank Marine Protected Area. This MPA exists offshore of Cape Breton Island, Nova Scotia, Canada. Samples were collected in August 2022. Sample depths ranged from approximately 25 metres to 230 metres.","sampling":"In August 2022, seawater samples were collected using 4 L Niskin bottles on an SBE 19plus CTD and SBE 55 rosette (Sea-Bird Scientific), closed at prescribed depths at regular intervals aboard the Canadian Coast Guard Ship M. Perley at multiple stations within the SAB MPA.\nNiskin bottles dedicated to eDNA filtration were also collected at the bottom and surface of each CTD rosette profile.  1 L water samples were frozen in Nalgene bottles until later thawing and filtration in a laboratory setting. The CTD also profiled depth, temperature, and conductivity at each sampling station. \n\nThe stations were sampled for eDNA to gain baseline and time series information on fish species in the MPA. Samples were filtered across a 0.22um PVDF Sterivex filter, which were shipped to the Centre for Environmental Genomics Applications (St. John's, Newfoundland). Samples were extracted in the laboratory, and libraries were prepared for the three eDNA markers (12S, 16S, and COI) for high-depth sequencing on the Illumina NovaSeq platform.","methodSteps":["The eDNA filters from 2022 were shipped on ice to the Centre for Environmental Genomics Applications (St. John’s, Newfoundland) for DNA extraction and sequencing. Briefly, each Sterivex filter was removed from its housing and digested in lysis buffer. Captured DNA is extracted from each filter, after which the DNA undergoes PCR amplification for the specific marker region being targeted. Nucleotide barcodes are added to the DNA from each filter to identify the sample during bioinformatic processing, and Illumina adapters are also added to enable next-generation sequencing on an Illumina platform. Amplified, barcoded DNA from each sample is then pooled into a sequencing library and sequenced on an Illumina instrument. In 2021 and 2022, libraries were sequenced at CEGA on an Illumina NovaSeq 6000 machine with a sequencing depth target of 1 million reads per sample.\nRaw sequence reads were converted to fastq format and demultiplexed. Forward and reverse primers were trimmed from all sequences using cutadapt v.4.0 (Martin 2011) with an error rate of 0.11, minimum quality score of 20, and minimum length set to 20. Demultiplexed reads were processed using the QIIME2-2024.10 pipeline (Boyen et al. 2019). Paired-end reads were imported for each marker separately using the import tool, and denoised using the DADA2 plugin. For 16S, –p-trunc-len-f and –p-trunc-len-r were both set to 125, but were set to 116 and 108 respectively for 12S. A consensus method was used to detect chimeras, and the –p-min-fold-parent-over-abundance parameter was set to 2 to improve the number of reads passing the chimera filter. Following denoising, the results were summarized as feature-tables including the filtered amplicon sequence variant (ASV) feature table, representative fasta sequences, and denoising statistics using the feature-table plugin. The taxonomy of each ASV sequence was assigned using BLASTn against the eukaryotic nucleotide database for 12S and 16S markers, while a Ribosomal Database Project (RDP) naive Bayesian classifier v5.1.0 was used to assign the taxonomy for the COI marker (Porter et al. 2014; Porter and Hajibabaei 2018). \nASV tables for each eDNA marker consisting of species names and read counts per species per eDNA sample were filtered for taxonomic identifications with a percent match <98%, and for non-aquatic species (e.g., terrestrial mammals, insects, fungi). Following data filtration, barplots of confirmed species and their read counts were created for each marker and each year of data. Species accumulation curves and non-metric multidimensional scaling (nMDS) plots were conducted in the vegan R package (Oksanen et al. 2025) to show species diversity and sample community similarities. Jaccard dissimilarities were used to create the distance matrix for the nMDS of the eDNA communities."]},"countryCoverage":[],"collections":[],"dataDescriptions":[],"dataLanguage":"eng","purpose":"Beginning in 2021 and to the present (2026), Fisheries and Oceans Canada has been monitoring St. Anns Bank Marine Protected Area as part of its mandate to expand marine conservation areas across Canada. In this study, we collected eDNA samples from a Canadian Coast Guard ship the M. Perley at a series of stations within the MPA. DNA extraction and sequencing took place at the Centre for Environmental Genomics Applications (St. John's, Newfoundland), which targeted three eDNA markers (12S, 16S, and COI). This work forms part of a baseline of fish and invertebrate communities in the MPA to examine stability or change in species richness and community structure over time.","acknowledgements":"This work was funded by the Marine Conservation Targets program at Fisheries and Oceans Canada.","pubDate":"2026-02-27T00:00:00.000+00:00","maintenanceUpdateFrequency":"AS_NEEDED","maintenanceDescription":"Separate resources will be developed for other years of eDNA data for SAB MPA. This resource will be updated as needed.","license":"http://creativecommons.org/publicdomain/zero/1.0/legalcode","dwca":{"coreType":"http://rs.tdwg.org/dwc/terms/Occurrence","extensions":["http://rs.gbif.org/terms/1.0/DNADerivedData","http://rs.iobis.org/obis/terms/ExtendedMeasurementOrFact"],"modified":"2026-07-13T14:01:32.331+00:00"},"category":["eDNA"],"approximateCounts":{"occurrenceCount":86260}}