The frozen collection consists of over 3,000 transgenic and mutant strains of non-parasitic nematodes, Caenorhabditis elegans species, accumulated over forty years. The collection, established in the early ’80s by Dr. Paolo Bazzicalupo, is kept in triplicate, is unique in Italy, and supports numerous Italian groups. C. elegans is the only multicellular animal model that can be frozen and stabs are stable for decades.
Preservation techniques: Cryopreservation of L1 larval stage worms in 1 mL freezing solution containing NaCl 0.1M, phosphate buffer pH 6.2, Glycerol 30%. Services: Distribution of strains to the Italian C. elegans community. Local conservation of strains from CAENORHABDITIS GENETICS CENTER (CGC). Genotyping and phenotyping.
Competence / Expertise: Maintenance of C. elegans strains, Genetic crosses, Molecular biology, Anatomical methods, Neurobiology, Toxicology, Implementation of nutraceuticals, Genetic screens, Drug screens, Transgenesis.
Quality: Genotyping and phenotyping of strains before and after freezing, including viability test. The collection is maintained as triplicate copies in 2 different -80° Ultrafreezers and 1 in L.N.
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SAC.AD002.173","contacts":[{"type":"PRINCIPAL_INVESTIGATOR","primary":false,"userId":["https://orcid.org/0000-0002-8179-6666"],"firstName":"Elia","lastName":"Di Schiavi","position":[],"email":[],"phone":[],"homepage":[],"address":[]}],"funding":"ITINERIS project no. IR0000032 — ITINERIS, Italian Integrated Environmental Research Infrastructures System—CUP B53C22002150006 (D.D. n. 130/2022) Funded by the EU—Next Generation EU—Mission 4 “Education and Research”—Component 2: “From research to business”—Investment 3.1: “Fund for the realisation of an integrated system of research and innovation infrastructures” || BIOMEMORY (CNR-DiSBA Project No. SAC.AD002.173","awards":[{"funderName":"Consiglio Nazionale delle Ricerche","funderIdentifiers":["https://doi.org/10.13039/501100004462"],"awardNumber":"CNR-DiSBA no. 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Farmaci e reti Biotecnologiche di Qualità\" from the Italian Ministry of Economy","contacts":[{"type":"PRINCIPAL_INVESTIGATOR","primary":false,"userId":["https://orcid.org/0000-0002-8179-6666"],"firstName":"Elia","lastName":"Di Schiavi","position":[],"email":[],"phone":[],"homepage":[],"address":[]}]},{"title":"Grant \"BANDO RICERCA FINALIZZATA 2009\" RF-2009-1475235 from the Italian Ministry of Health","contacts":[{"type":"PRINCIPAL_INVESTIGATOR","primary":false,"userId":["https://orcid.org/0000-0002-8179-6666"],"firstName":"Elia","lastName":"Di Schiavi","position":[],"email":[],"phone":[],"homepage":[],"address":[]}]},{"title":"Grant Misura 3.17-2008 from Regione Campania, Italy","contacts":[{"type":"PRINCIPAL_INVESTIGATOR","primary":false,"userId":["https://orcid.org/0000-0002-8179-6666"],"firstName":"Elia","lastName":"Di Schiavi","position":[],"email":[],"phone":[],"homepage":[],"address":[]}]},{"title":"Grant Exploratory Project GEP14141 from Italian Telethon Foundation","contacts":[{"type":"PRINCIPAL_INVESTIGATOR","primary":false,"userId":["https://orcid.org/0000-0002-8179-6666"],"firstName":"Elia","lastName":"Di Schiavi","position":[],"email":[],"phone":[],"homepage":[],"address":[]}]},{"title":"Grant \"BANDO RICERCA SCIENTIFICA E TECNOLOGICA 2015\" 2015.03003.021 from Fondazione Cassa di Risparmio di Perugia, Italy","contacts":[{"type":"PRINCIPAL_INVESTIGATOR","primary":false,"userId":["https://orcid.org/0000-0002-8179-6666"],"firstName":"Elia","lastName":"Di Schiavi","position":[],"email":[],"phone":[],"homepage":[],"address":[]}]},{"title":"Grant \"BeHEARD Technology Prizes Rare Disease Challenge\"","contacts":[{"type":"PRINCIPAL_INVESTIGATOR","primary":false,"userId":["https://orcid.org/0000-0002-8179-6666"],"firstName":"Elia","lastName":"Di Schiavi","position":[],"email":[],"phone":[],"homepage":[],"address":[]}]},{"title":"Grant Full Project GGP16203 from Italian Telethon Foundation","contacts":[{"type":"PRINCIPAL_INVESTIGATOR","primary":false,"userId":["https://orcid.org/0000-0002-8179-6666"],"firstName":"Elia","lastName":"Di Schiavi","position":[],"email":[],"phone":[],"homepage":[],"address":[]}]},{"title":"Grant H2020-FETOPEN-1-2016-2017 801338 from European Community","contacts":[{"type":"PRINCIPAL_INVESTIGATOR","primary":false,"userId":["https://orcid.org/0000-0002-8179-6666"],"firstName":"Elia","lastName":"Di Schiavi","position":[],"email":[],"phone":[],"homepage":[],"address":[]}]},{"title":"Grant Joint Bilateral Agreement CNR/RFBR from National Research Council of Italy (CNR)","contacts":[{"type":"PRINCIPAL_INVESTIGATOR","primary":false,"userId":["https://orcid.org/0000-0002-8179-6666"],"firstName":"Elia","lastName":"Di Schiavi","position":[],"email":[],"phone":[],"homepage":[],"address":[]}]},{"title":"Grant SMA-Europe Operating Grant 2019 22757","contacts":[{"type":"PRINCIPAL_INVESTIGATOR","primary":false,"userId":["https://orcid.org/0000-0002-8179-6666"],"firstName":"Elia","lastName":"Di Schiavi","position":[],"email":[],"phone":[],"homepage":[],"address":[]}]},{"title":"Grant H2020-FETOPEN-PROACTIVE-EIC-05 NUMBER 952183 from European Community","contacts":[{"type":"PRINCIPAL_INVESTIGATOR","primary":false,"userId":["https://orcid.org/0000-0002-8179-6666"],"firstName":"Elia","lastName":"Di Schiavi","position":[],"email":[],"phone":[],"homepage":[],"address":[]}]},{"title":"Grant CHARGE Syndrome Foundation Research Grant Program 2021 from Charge Foundation","contacts":[{"type":"PRINCIPAL_INVESTIGATOR","primary":false,"userId":["https://orcid.org/0000-0002-8179-6666"],"firstName":"Elia","lastName":"Di Schiavi","position":[],"email":[],"phone":[],"homepage":[],"address":[]}]},{"title":"Research Grant AFM-TELETHON ANNUAL CALL FOR PROPOSALS 2022 from AFM-Telethon France","contacts":[{"type":"PRINCIPAL_INVESTIGATOR","primary":false,"userId":["https://orcid.org/0000-0002-8179-6666"],"firstName":"Elia","lastName":"Di Schiavi","position":[],"email":[],"phone":[],"homepage":[],"address":[]}]}],"abstract":"ITINERIS (PNRR Project IR0000032) Activity 6.5 \"Mining and mapping the functional biodiversity in vivo and ex-situ research collections\" - This activity is aimed to provide biodiversity (meta)data linked to Natural Science Collections (NSC) in terms of extended digital specimens fully linked to related information (e.g.. images and videos, codification of functional traits, DNA and protein sequences, environmental parameters, etc.). The expected outcome of the activity is the implementation of a national network of biological research collections, including all the existing in vivo and ex-situ collections of biological organisms (animals, plants, fungi, algae, archaea, bacteria, viruses, etc.) which have been created and maintained through past and current research projects. The full digitization of the research collections currently offline will be achieved along with the reorganization, standardization and harmonization of the data and metadata currently available in the main online collections. All the taxonomic, genetic, geographical, ecological, physiological information for each specimen will be organized in an easily searchable platform supported by web-GIS functionalities. Reference to genetic/genomic data for each specimen will allow for the identification of the most interesting material for further genetic/genomic analysis. The availability of such huge amount of information on plant, animal, and microbial genetic resources will pave the way for the dissection of the morpho-physiological and molecular mechanisms of adaptation to climate change. || BIOMEMORY (CNR-DiSBA Project No. SAC.AD002.173) is the network of biological collections of the Department of Biology, Agriculture and Food Sciences (DiSBA) of the National Research Council of Italy (CNR) for bio-monitoring, biodiversity conservation, agri-food and environmental sustainability, and human well-being. The project is aimed to create a network of biobanks (i.e., scientific research collections) where data and metadata associated to biological samples of different nature are collected and stored in a systematic and well-organized way. Maintaining the existing collections will allow their future use for a number of purposes, from the genetic improvement of organisms to face environmental changes (climate-ready organisms) to the fight against epidemics and pandemics affecting humans, animals and plants."},"countryCoverage":[],"collections":[{"name":"Caenorhabditis elegans Italian collection (CelITAbase)","identifier":"1d38cf8c-78a6-411e-b574-533dc840b43c","specimenPreservationMethod":"DEEP_FROZEN","curatorialUnits":[]}],"dataDescriptions":[{"name":"API-JSON","charset":"UTF-8","url":"https://biomemory.cnr.it/api/occurrences/json/?dataset=141","format":"DwC"}],"dataLanguage":"eng","purpose":"Not-for-profit use only. Without our written consent, all strains may not be redistributed outside the recipient’s organization. Some strains can be distributed by accepting to cite the paper in which it has been described. Some strains contain patented and/or exclusively licensed components and can be distributed only under a collaborative agreement.
","acknowledgements":"PNRR Project IR0000032—ITINERIS, Italian Integrated Environmental Research Infrastructures System—CUP B53C22002150006 (D.D. n. 130/2022) Funded by the EU—Next Generation EU—Mission 4 “Education and Research”—Component 2: “From research to business”—Investment 3.1: “Fund for the realization of an integrated system of research and innovation infrastructures”
CNR-DiSBA Project No. SAC.AD002.173 BioMemory - Network of scientific collections for bio-monitoring, biodiversity conservation, agri-food and environmental sustainability, and well-being.
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