10.15468/hhkhz2
0ea51b6e-d02f-495e-a29b-73783e4060c0
https://ipt.biodiversity.aq/resource?r=methanobasealaska
Bacteria and Archaea biodiversity in Arctic and Subarctic terrestrial ecosystems in Alaska
Maialen
Barret
Laboratoire Ecologie Fonctionnelle et Environnement (UMR5245, Université de Toulouse, France)
Associate professor
Toulouse
FRANCE
maialen.barret@ensat.fr
Frederic
Thalasso
Cinvestav
Mexico City
MEXICO
Laure
Gandois
Laboratoire Ecologie Fonctionnelle et Environnement (UMR5245, Université de Toulouse, France)
Toulouse
FRANCE
Klara
Martinez Cruz
Universidad de Magallanes
Punta Arenas
CHILE
Armando
Sepulveda Jaureguy
Universidad de Magallanes
Punta Arenas
CHILE
Céline
Lavergne
Pontificia Universidad Católica de Valparaíso
Valparaiso
CHILE
Roman
Teisserenc
Laboratoire Ecologie Fonctionnelle et Environnement (UMR5245, Université de Toulouse, France)
Researcher
Toulouse
FRANCE
Polette
Aguilar
Pontificia Universidad Católica de Valparaíso
Valparaíso
CHILE
Oscar
Gerardo-Nieto
Cinvestav
Mexico City
MEXICO
Claudia
Etchebehere
Instituto de Investigaciones Biológicas Clemente Estable
Montevideo
URUGUAY
Bruna
Martins
Instituto de Investigaciones Biológicas Clemente Estable
Montevideo
URUGUAY
Javier
Fochesatto
University of Alaska Fairbanks
Fairbanks
UNITED_STATES
Nikita
Tananaev
P.I. Melnikov Permafrost Institute
Igarka
RUSSIAN_FEDERATION
Mette
Svenning
UiT The Arctic University of Norway
Tromso
NORWAY
Christophe
Seppey
UiT The Arctic University of Norway
Tromso
NORWAY
Alexander
Tveit
UiT The Arctic University of Norway
Tromso
NORWAY
Rolando
Chamy
Pontificia Universidad Católica de Valparaíso
Valparaíso
CHILE
Maria
Soledad Astorga-España
Universidad de Magallanes
Punta Arenas
CHILE
Andres
Mansilla
Universidad de Magallanes
Punta Arenas
CHILE
Anton
Van de Putte
Royal Belgian Institute for Natural Sciences
Brussels
BELGIUM
Maxime
Sweetlove
Royal Belgian Institute for Natural Sciences
Brussels
BELGIUM
Alison
Murray
Desert Research Institute
Reno
UNITED_STATES
Léa
Cabrol
Institut Méditerranéen d’Océanologie
Researcher
Marseille
FRANCE
lea.cabrol@mio.osupytheas.fr
Maialen
Barret
Laboratoire Ecologie Fonctionnelle et Environnement (UMR5245, Université de Toulouse, France)
Associate professor
Toulouse
FRANCE
maialen.barret@ensat.fr
Maialen
Barret
maialen.barret@ensat.fr
USER
2022-04-15
ENGLISH
Methane emissions from aquatic and terrestrial ecosystems play a crucial role in global warming, which is particularly affecting high-latitude ecosystems. As major contributors to methane emissions in natural environments, the microbial communities involved in methane production and oxidation deserve a special attention. Microbial diversity and activity are expected to be strongly affected by the already observed (and further predicted) temperature increase in high-latitude ecosystems, eventually resulting in disrupted feedback methane emissions. The METHANOBASE project has been designed to investigate the intricate relations between microbial diversity and methane emissions in Arctic, Subarctic and Subantarctic ecosystems, under natural (baseline) conditions and in response to simulated temperature increments. We report here a small subunit ribosomal RNA (16S rRNA) analysis of lake, peatland and mineral soil ecosystems.
metadata
GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml
16S rRNA
metabarcoding
bacteria
procaryote
wetland
peatland
soil
MiSeq
sediment
greenhouse gas
archaea
methane
lake
n/a
Metadata
GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml
This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
Creative Commons Attribution 4.0 International
https://spdx.org/licenses/CC-BY-4.0.html
CC-BY-4.0
Alaska
-150.8
-147.65
68.62
63.21
2016-06-27
2016-06-27
Bacterial and Archaea diversity was profiled by targeting the V4-V5 region of the 16S SSU rRNA gene for high throughput metabarcode (amplicon) sequencing, using the Illumia MiSeq platform (2x 250bp).
Domain
Bacteria
Bacteria
Domain
Archaea
Archaea
unkown
Maialen
Barret
Laboratoire Ecologie Fonctionnelle et Environnement (UMR5245, Université de Toulouse, France)
Associate professor
Toulouse
FRANCE
maialen.barret@ensat.fr
Léa
Cabrol
Institut méditerranéen d'Océanologie
Researcher
Marseille
FRANCE
lea.cabrol@mio.osupytheas.fr
After collection, samples were stored at 4°C prior to further processing. Liquid samples were filtered at 0.45µm until clogging and the filters were stored at -20°C. DNA was extracted from these filters using the PowerWater DNA isolation kit (MOBIO) while DNA was extracted from solid samples using the PowerSoil DNA isolation kit (MOBIO). DNA extracts were kept at -20°C. The V4-V5 region of 16S rRNA gene was amplified in the following conditions: 515F and 928R primers (Wang & Qian, 2009. doi:10.1371/journal.pone.0007401), 2min at 94°C, 30 cycles of 60s at 94°C, 40s at 65°C and 30s at 72°C, and 10 min at 72°C. Amplicon sequencing was carried out with Illumina MiSeq technology (2x250pb, V3). Denoising of the sequences dataset and OTU clustering was carried using the FROGS pipeline (Auer et al., 2017. doi:10.1093/bioinformatics/btx791). BLAST was used for taxonomic affiliation.
Samples were collected in summer 2015, without any temporal replication.
A total of 19 ecosystems were studied in Alaska, USA. The selected sites are representative of this Subantarctic region: lakes, peatlands, Nothofagus forest, pampa
In each site, various samples were collected to take into account the local heterogeneity: different depths in water column and sediments, soil horizons, hollows/edges/hummocks.
Water samples were collected with a Van Dorn bottle. Sediments were sampled thanks to a grab-sampler, peat monoliths (approximately 30*30*30cm) were cut with a bread-knife and soil monoliths with a shovel.
Methanobase
Maialen
Barret
ADMINISTRATIVE_POINT_OF_CONTACT
METHANOgenic Biodiversity and activity in Arctic, subarctic and Subantarctic Ecosystems affected by climate change
ERANET-LAC joint call 2014
Alaska
Lakes (water, sediments), peatlands (hollows, edges, hummocks) and mineral soils
The METHANOBASE project has been designed to investigate the intricate relations between microbial diversity and methane emissions in Arctic, Subarctic and Subantarctic ecosystems, under natural (baseline) conditions and in response to simulated temperature increments.
2026-04-10T09:54:02Z
Barret M, Thalasso F, Gandois L, Martinez Cruz K, Sepulveda Jaureguy A, Lavergne C, Teisserenc R, Aguilar P, Gerardo-Nieto O, Etchebehere C, Martins B, Fochesatto J, Tananaev N, Svenning M, Seppey C, Tveit A, Chamy R, Soledad Astorga-España M, Mansilla A, Van de Putte A, Sweetlove M, Murray A, Cabrol L (2022). Bacteria and Archaea biodiversity in Arctic and Subarctic terrestrial ecosystems in Alaska. Version 1.4. SCAR - Microbial Antarctic Resource System. Metadata dataset https://doi.org/10.15468/hhkhz2 accessed via GBIF.org on 2026-04-10.
http://ipt.biodiversity.aq/logo.do?r=methanobasealaska