10.15468/hnlivf https://ipt.biodiversity.aq/resource?r=fungi_soils_s_shetland_and_antarctica 11c8e968-361d-4cea-abae-67450fa03fe8 http://ipt.biodiversity.aq/resource?r=fungi_soils_s_shetland_and_antarctica Marcelo Baeza Universidad de Chile

Santiago CHILE

Salvador Barahona Universidad de Chile

Santiago CHILE

Jennifer Alcaino Universidad de Chile

Santiago CHILE

Victor Cifuentes Universidad de Chile

Santiago CHILE

Maxime Sweetlove Royal Belgian Institute for Natural Sciences Research assistent

Rue Vautier 29 Brussels 1000 BELGIUM

msweetlove@naturalsciences.be 2019-03-19 ENGLISH Amplicon sequencing dataset of microbial Fungi (LSU D1-D2) of terrestrial habitats in Antarctica, including eight islands of the South Shetland Archipelago, two islands on the Antarctic Peninsula and Union Glacier. Metadata GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License. Creative Commons Attribution 4.0 International https://spdx.org/licenses/CC-BY-4.0.html CC-BY-4.0 South Shetland Islands, Union Glacier (Antarctica) -83.31 -58.1 -62.1 -79.82 2010 2015 LSU marker gene for microbial soil Fungi Phylum Fungi Fungi unkown Marcelo Baeza Universidad de Chile

Santiago CHILE

A PowerSoil DNA Isolation Kit (MO BIO Laboratories Inc., Carlsbad, CA, United States) was used for direct DNA extraction from soil samples. The manufacturers’ instructions were followed, with only one modification, the disruption step, was performed using a Mini Beadbeater-16 cell disrupter (BioSpec Bartlesville, United States) instead of vortex agitation, as no PCR-amplicons were obtained in reactions using samples obtained through vortex agitation. The PCR reactions were performed using 1 μl of DNA sample (direct or 1/10 dilution), 5 units of Taq DNA polymerase, dNTP mix at 0.4 mM each, forward and reverse primers at 1 mM final each, PCR buffer and MgCl2 2 mM. Amplification was performed using a 2720 (Applied Biosystems) thermal cycler using the following protocol: initial denaturation at 94°C for 3 min; 35 cycles of denaturation at 94°C for 30 s, annealing at 50°C for 3 min, and extension at 72°C for 3 min; and a final extension step at 72°C for 10 min. The universal primers F63 (5′-GCA TAT CAA TAA GCG GAG GAA AAG-3′) and LR3 (5′-GGT CCG TGT TTC AAG ACG G-3′) were used; these primers have been described as specific for amplifying the fungal D1/D2 region of the large subunit ribosomal gene (LSU). In all PCR reactions, the same forward primer (F63) was used, but the reverse primer (LR3) was specific for each soil sample as a 454 adapter, a specific barcode and a linker sequence were added. Sequencing was performed at OMICS Solutions (Santiago, Chile) using the Ion 314TM Chip Kit v2 (Thermo Fisher) and Ion Torrent personal genome machine (PGM), according to the manufacturer’s instructions (Rothberg et al., 2011). Three independent runs were performed: (i) samples from King George Island; (ii) samples from Deception, Snow, Dee, Livingstone, Greenwich, Robert, Nelson and Litchfield islands; and iii) samples from Lagotellerie and Union Glacier. Soil samples were gathered during several expeditions to Antarctica, including Union Glacier, Lagotellerie island, King George, Deception, Snow, Dee, Livingstone, Greenwich, Robert, Nelson and Litchfield islands. Samples were collected in sterile 50 ml plastic tubes, which were sealed and shipped at -20°C to the Genetics Laboratory of the Faculty of Science at the Universidad de Chile. Once samples arrived at the laboratory, they were maintained at -80°C until processing. Marcelo Baeza ADMINISTRATIVE_POINT_OF_CONTACT Salvador Barahona ADMINISTRATIVE_POINT_OF_CONTACT Jennifer Alcaino ADMINISTRATIVE_POINT_OF_CONTACT Victor Cifuentes ADMINISTRATIVE_POINT_OF_CONTACT This work was supported by Comisión Nacional de Investigación Científica y Tecnológica (No. FONDECYT grant 1130333). 2026-04-10T09:54:02Z Baeza M, Barahona S, Alcaino J, Cifuentes V, Sweetlove M (2019). Fungi (LSU) in soils from the South Shetland island, Antarctic Peninsula island, and Union Glacier. Version 1.3. SCAR - Microbial Antarctic Resource System. Metadata dataset https://doi.org/10.15468/hnlivf accessed via GBIF.org on 2026-04-10. Baeza, M., Barahona, S., Alcaíno, J., & Cifuentes, V. (2017). Amplicon-Metagenomic Analysis of Fungi from Antarctic Terrestrial Habitats. Frontiers in microbiology, 8, 2235. Carrasco, M., Rozas, J. M., Barahona, S., Alcaíno, J., Cifuentes, V., & Baeza, M. (2012). Diversity and extracellular enzymatic activities of yeasts isolated from King George Island, the sub-Antarctic region. BMC microbiology, 12(1), 251. Troncoso, E., Barahona, S., Carrasco, M., Villarreal, P., Alcaíno, J., Cifuentes, V., & Baeza, M. (2017). Identification and characterization of yeasts isolated from the South Shetland Islands and the Antarctic Peninsula. Polar Biology, 40(3), 649-658. Barahona, S., Yuivar, Y., Socias, G., Alcaíno, J., Cifuentes, V., & Baeza, M. (2016). Identification and characterization of yeasts isolated from sedimentary rocks of Union Glacier at the Antarctica. Extremophiles, 20(4), 479-491.