10.15468/y7hujy https://ipt.biodiversity.aq/resource?r=eukaryotes_bacteria_in_wetted_vs_arid_antarctic_dry_valley_soils 55ed602c-dc49-41d2-a860-4a626ba85ba8 http://ipt.biodiversity.aq/resource?r=eukaryotes_bacteria_in_wetted_vs_arid_antarctic_dry_valley_soils Thomas Niederberger University of Delaware

Lewes UNITED_STATES

Jill Sohm University of Southern California

Los Angeles UNITED_STATES

Troy Gunderson University of Southern California

Los Angeles UNITED_STATES

Alexander Parker San Francisco State Universit

Tiburon UNITED_STATES

Joëlle Tirindelli San Francisco State Universit

Tiburon UNITED_STATES

Douglas Capone University of Southern California

Los Angeles UNITED_STATES

Edward Carpenter San Fransisco State University

Tiburon UNITED_STATES

Stephen Cary University of Delaware

Lewes UNITED_STATES

Maxime Sweetlove Royal Belgian Institute for Natural Sciences Research assistent

Rue Vautier 29 Brussels 1000 BELGIUM

msweetlove@naturalsciences.be 2019-03-19 ENGLISH Amplicon sequencing dataset of Bacteria (16S ssu rRNA gene) and Eukaryotes (18S ssu rRNA gene) from wet and dry soil samples near Miers stream, Nostoc pond, Miers lake and Buddha lake, in the McMurdo Dry Valleys, Antarctica. Metadata GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License. Creative Commons Attribution 4.0 International https://spdx.org/licenses/CC-BY-4.0.html CC-BY-4.0 Sample pairs were taken from wet and dry soil near Miers stream, Nostoc pond, Miers lake and Buddha lake, in the McMurdo Dry Valleys, Antarctica. 163.747 163.832 -78.06 -78.096 2009-01-18 2009-12-08 Eukaryote (18S ssu rRNA marker gene) and Bacterial (16S ssu rRNA marker gene) microorganisms Domain Eukaryota Eukaryotes Domain Bacteria Bacteria unkown Thomas Niederberger University of Delaware

Lewes UNITED_STATES

Stephen Cary University of Delaware

Lewes UNITED_STATES

DNA was isolated from homogenized soil samples using the PowersoilTM DNA isolation kit (MOBIO) as per manufacturer’s instructions with all terminal-restriction fragment length polymorphisms (T-RFLPs) based on pooled triplicate PCR assays. Tagged amplicon pyrosequencing of the V1 to V3 region of the 16S and 18S rRNA gene (primer pairs 28F-519R and euk7F-euk570R, respectively) was performed on DNA extracts by the Research and Testing Laboratories located in Lubbock, TX (www.researchandtesting.com) using FLX technology (Roche). Samples were collected from the Miers Valley region of Antarctica during the summer months, including: MS1, Miers Stream 1 (S78°05.748′, E163°44.822′), a site located at the edge of the runoff stream flowing from Miers Glacier into Miers Lake in Miers Valley; ML1, Miers Lake 1 (S78°05.615′, E163°49.912′) located on the north side of Miers Lake; BL, Baby Buddha Lake (S78°03.621′, E163°46.463′), and NP, Nostoc Pond (S78°03.920′, E163°46.497′) adjacent to lake systems located over the north ridge of Miers Valley. Soil samples were collected aseptically at each site into sterile Whirl-Pak bags using a sterile metal spatula and kept frozen during transport to the laboratory. Douglas Capone ADMINISTRATIVE_POINT_OF_CONTACT Edward Carpenter ADMINISTRATIVE_POINT_OF_CONTACT Stephen Cary ADMINISTRATIVE_POINT_OF_CONTACT This research was supported by National Science Foundation Grants ANT 0739633, ANT 0739640, and ANT 0739648 and 0944560. 2026-04-26T08:29:02Z Niederberger T, Sohm J, Gunderson T, Parker A, Tirindelli J, Capone D, Carpenter E, Cary S, Sweetlove M (2019). Microbial communities (Eukaryotes and Bacteria) of transiently wetted vs. arid Antarctic Dry Valley soils. Version 1.2. SCAR - Microbial Antarctic Resource System. Metadata dataset https://doi.org/10.15468/y7hujy accessed via GBIF.org on 2026-04-26. Niederberger, T. D., Sohm, J. A., Gunderson, T. E., Parker, A. E., Tirindelli, J., Capone, D. G., ... & Cary, S. C. (2015). Microbial community composition of transiently wetted Antarctic Dry Valley soils. Frontiers in microbiology, 6, 9. Niederberger, T. D., Sohm, J. A., Tirindelli, J., Gunderson, T., Capone, D. G., Carpenter, E. J., & Cary, S. C. (2012). Diverse and highly active diazotrophic assemblages inhabit ephemerally wetted soils of the Antarctic Dry Valleys. FEMS microbiology ecology, 82(2), 376-390.