{"key":"6789ac99-6532-4f2b-85bb-cb1e5add7a95","installationKey":"1de87984-ce7c-407c-988c-a28014ae53ad","publishingOrganizationKey":"e794e60e-e558-4549-99f8-cfb241cdce24","doi":"10.15468/wqp57u","external":false,"numConstituents":0,"type":"OCCURRENCE","title":"NEON Biorepository Benthic Macroinvertebrate Collection (Fluid Preserved Oligochaetes)","description":"<p>\r\nOligochaete (worm) bulk taxonomy sample identifications collected from wadeable streams, rivers, and lakes.\r\nCollected samples are separated into fluid-preserved general macroinvertebrates, fluid-preserved chironomids, fluid-preserved oligochaetes, and slide-mounted chironomids and oligochaetes. Taxonomic identifications are generated from subsamples of each group. This sample type contains taxonomic identifications derived from subsample analysis of fluid-preserved oligochaetes.\r\nSampling\r\n\r\n3 sampling bouts per year (spring, summer, fall)\r\n8 locations at each NEON site\r\n\r\n5 in dominant habitat type (wadeable stream) or littoral area (lake and river)\r\n3 in sub-dominant habitat type (wadeable streams) or pelagic area (lakes and rivers)\r\n\r\n\r\n11 samples collected per bout total\r\n\r\n8 morphology samples (1 per location)\r\n3 DNA samples (3 dominant/littoral locations)\r\n\r\nBouts 1 &amp;amp; 3 DNA samples archived unprocessed\r\nBout 2 samples extracted and sequenced\r\n\r\n\r\n\r\n\r\n\r\nMethods\r\n\r\nHabitat-appropriate sampling gear (e.g., Surber, Hess, corer, kicknet, D-frame sweep, petite ponar)\r\nSamples collected from natural substrate surface\r\n\r\nPreservation\r\n\r\nSamples shipped to taxonomist for sorting and identification into general macroinvertebrates, chironomids, and oligochaetes\r\n\r\nTaxonomic reference samples can be prepared and archived at the end of a taxonomist&#39;s contract period\r\n\r\n\r\nPreserved in 70-95% ethanol\r\nMaintained in temperature- and humidity-controlled storage\r\n\r\n</p>","language":"eng","citation":{"text":"National Ecological Observatory Network (2026). NEON Biorepository Benthic Macroinvertebrate Collection (Fluid Preserved Oligochaetes). 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