10.15468/pematf
https://ipt.biodiversity.aq/resource?r=antarctic_desert_surface_soil_microbiome
Antarctic polar desert surface soil microbiome
Mukan
Ji
Australian Centre for Astrobiology
Randwick
AUSTRALIA
Chris
Greening
Monash University
Clayton
AUSTRALIA
Inka
Vanwonterghem
University of Queensland
St Lucia
AUSTRALIA
Carlo
Carere
Wairakei Research Centre
Wairakei
NEW_ZEALAND
Sean
Bay
Monash University
Clayton
AUSTRALIA
Jason
Steen
University of Queensland
St. Lucia
AUSTRALIA
Kate
Montgomery
Australian Centre for Astrobiology
Randwick
AUSTRALIA
Thomas
Lines
Monash University
Clayton
AUSTRALIA
Josie
van Dorst
Australian Centre for Astrobiology
Randwick
AUSTRALIA
Ian
Snape
Australian Antarctic Division
Kingston
AUSTRALIA
Matthew
Stott
Wairakei Research Centre
Wairakei
NEW_ZEALAND
Philip
Hugenholtz
University of Queensland
St. Lucia
AUSTRALIA
Belinda
Ferrari
Australian Centre for Astrobiology
Randwick
AUSTRALIA
Maxime
Sweetlove
Royal Belgian Institute of Natural Sciences
Research assistent
Rue Vautier 29
Brussels
1000
BELGIUM
msweetlove@naturalsciences.be
2019-03-28
ENGLISH
Whole-genome shotgun sequencing dataset targeting microbes in three soil samples from pristine polar desert at Robinson Ridge (eastern Antarctica)
Metadata
GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml
This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
Creative Commons Attribution 4.0 International
https://spdx.org/licenses/CC-BY-4.0.html
CC-BY-4.0
Robinson Ridge, east Antarctica
110.586
110.586
-66.37
-66.37
2015-12-13
whole genome shotgun sequencing
Domain
Bacteria
Bacteria
Domain
Archaea
Archaea
Domain
Eukaryota
Eukaryotes
Domain
Viri
viruses
unkown
Mukan
Ji
Australian Centre for Astrobiology
Randwick
AUSTRALIA
Chris
Greening
Monash University
Clayton
AUSTRALIA
Belinda
Ferrari
Australian Centre for Astrobiology
Randwick
AUSTRALIA
Total community DNA was extracted from 0.25–0.3 g of each sample in technical triplicate using the FastDNA SPIN Kit for Soil (MP Biomedicals). All DNA extracts were quantified and DNA lysate quality was evaluated using automated ribosomal intergenic spacer analysis (ARISA).
Metagenome libraries were prepared using the Nextera DNA Library Preparation Kit (Illumina) and sequenced using three-fifths of an Illumina HiSeq2000 flowcell lane at the Institute for Molecular Biosciences (University of Queensland).
samples were taken at the ice free Robinson Ridge (−66.367739, 110.585262), located 10 km south of Casey station in the Windmill Islands coast of Wilkes Land, is part of a pristine polar desert. Three surface soil samples (100 g) from the top 10 cm of the soil profile were collected in December 2005.
Samples were collected along a spatially explicit sampling design comprised of three 300-m-long transects, separated by 2-m distances from each other. Samples were sieved down to 63 μm, aliquoted into 5–25-g subsamples, and stored at −80 °C until analysis.
Bioplatforms Australia
Mukan
Ji
ADMINISTRATIVE_POINT_OF_CONTACT
This work was supported by Bioplatforms Australia, an Australian Antarctic Science project grant (4406), an ARC Future Fellowship (FT170100341), an ARC DECRA Fellowship (DE170100310), an ARC DORA and Laureate Fellowship (DP120103498 and FL150100038), and a Marsden Grant (16-GNS-035). UNSW Sydney, the Centre for Geometric Biology (Monash University), and the Geothermal Resources of New Zealand research program (GNS Science) also provided funding to support this research.
2026-04-10T09:54:01Z
Ji M, Greening C, Vanwonterghem I, Carere C, Bay S, Steen J, Montgomery K, Lines T, van Dorst J, Snape I, Stott M, Hugenholtz P, Ferrari B, Sweetlove M (2019). Antarctic polar desert surface soil microbiome. SCAR - Microbial Antarctic Resource System. Metadata dataset https://doi.org/10.15468/pematf accessed via GBIF.org on 2026-04-10.
Ji, M., Greening, C., Vanwonterghem, I., Carere, C. R., Bay, S. K., Steen, J. A., ... & Snape, I. (2017). Atmospheric trace gases support primary production in Antarctic desert surface soil. Nature, 552(7685), 400.