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Five different biomarkers were analysed, including 12SMifish, Mimammal, Teleo, 16S-Vert and COI markers. The sequences were analysed with the PacMAN pipeline and quality controlled including a validation check by local scientists and site managers as well as compared to existing knowledge on species distributions in OBIS and GBIF. The full sampling protocols and materials are available on the project website (https://www.unesco.org/en/edna-training-materials?hub&#61;66910), and the bioinformatics pipeline is on github (https://github.com/iobis/PacMAN-pipeline). The data can also be explored on the eDNA expeditions dashboard (https://dashboard.ednaexpeditions.org/).</p>","language":"eng","citation":{"text":"Melesio Tiscareño Villorin J, Erendida Frías Hernández L, Hernández González O, Rivera Reyes N, Berumen Solórzano P, Vargas Cossio P, Ávila García B, Guadalupe Villavicencio Rousseau G, López Payán F, Munguia Vega A, Manuel Duarte Ramos F, Carlos Fernando San Esteban Belmar J, Suominen S, Provoost P, Gillard E, Douvere F, Appeltans W (2026). UNESCO eDNA expedition in Archipiélago de Revillagigedo (Mexico): April 2023. Version 2.1. OBIS Secretariat. 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