{"key":"f9b0af39-cd0a-4d88-b9a6-3ec5ac7dae3f","installationKey":"5ff1c992-5c84-40aa-bd9a-c1743a870c32","publishingOrganizationKey":"c98a8d0d-cab2-4506-8c0f-c91a5e4996b9","doi":"10.15468/jhrruj","version":"1.2","external":false,"numConstituents":0,"type":"OCCURRENCE","shortName":"Coastal ESI eDNA 2023","title":"Coastal Biodiversity Monitoring in the Eastern Shore Islands Area of Interest using Environmental DNA - 2023","description":"In this project we conduct seasonal beach seining and eDNA sampling in the Eastern Shore Islands, a coastal "area of interest" in Nova Scotia, Canada. The records contained here include eDNA metabarcoding results from the 12S (targeting fish) and COI (targeting general biodiversity) markers. At each of five coastal sampling stations we conduct beach seining to catch and measure fish, and collect water samples for eDNA metabarcoding. Beach seining and eDNA can complement one another, since neither method will capture every species found at the sampling sites on their own. ","language":"eng","citation":{"text":"Jeffery N, Stanley R, Pettitt-Wade H (2026). Coastal Biodiversity Monitoring in the Eastern Shore Islands Area of Interest using Environmental DNA - 2023. Version 1.2. Fisheries and Oceans Canada. Occurrence dataset https://doi.org/10.15468/jhrruj accessed via GBIF.org on 2026-10-14.","citationProvidedBySource":false},"contactsCitation":[{"key":6918152,"abbreviatedName":"Jeffery N","firstName":"Nick","lastName":"Jeffery","roles":["ORIGINATOR"],"userId":[]},{"key":6918153,"abbreviatedName":"Stanley R","firstName":"Ryan","lastName":"Stanley","roles":["ORIGINATOR"],"userId":[]},{"key":6918154,"abbreviatedName":"Pettitt-Wade H","firstName":"Harri","lastName":"Pettitt-Wade","roles":["ORIGINATOR"],"userId":[]}],"lockedForAutoUpdate":false,"createdBy":"c98a8d0d-cab2-4506-8c0f-c91a5e4996b9","modifiedBy":"jwaller","created":"2026-02-18T18:05:20.467+00:00","modified":"2026-02-23T09:53:35.860+00:00","contacts":[{"key":6918152,"type":"ORIGINATOR","primary":true,"userId":[],"firstName":"Nick","lastName":"Jeffery","position":[],"email":["nick.jeffery@dfo-mpo.gc.ca"],"phone":[],"homepage":[],"organization":"Fisheries and Oceans Canada","address":[],"city":"Dartmouth","province":"Nova 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were collected from several sampling sites along Nova Scotia's Eastern Shore. The sample sites range from Conrod Beach in the west, to Goldboro in the east.","boundingBox":{"minLatitude":44.699743,"maxLatitude":45.165722,"minLongitude":-63.179877,"maxLongitude":-61.643597,"globalCoverage":false}}],"temporalCoverages":[{"@type":"range","start":"2023-05-01T00:00:00.000+00:00","end":"2023-11-30T00:00:00.000+00:00"}],"keywordCollections":[{"thesaurus":"GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml","keywords":["Occurrence"]}],"project":{"title":"Coastal biodiversity monitoring in the Eastern Shore Islands Area of Interest using Environmental DNA Metabarcoding - 2023","contacts":[{"type":"ORIGINATOR","primary":false,"userId":["https://orcid.org/0000-0003-4242-5712"],"firstName":"Nick","lastName":"Jeffery","position":[],"email":[],"phone":[],"homepage":[],"address":[]},{"type":"ORIGINATOR","primary":false,"userId":[],"firstName":"Ryan","lastName":"Stanley","position":[],"email":[],"phone":[],"homepage":[],"address":[]}],"funding":"This work was funded by the Fisheries and Oceans Canada's Marine Conservation Targets program.","awards":[],"studyAreaDescription":"The study area includes the coastal marine zone and intertidal area of the Eastern Shore Islands Area of Interest in Nova Scotia, Canada. Sampling sites include Conrods Beach, Little Harbour, Wolfes Island, Taylor Head Provincial Park, Moosehead Cove, and Goldboro. Each sample site includes an eelgrass meadow (Zostera marina) in a shallow bay.","relatedProjects":[]},"samplingDescription":{"studyExtent":"The study area includes the coastal marine zone and intertidal area of the Eastern Shore Islands Area of Interest in Nova Scotia, Canada. Sampling sites include Conrods Beach, Little Harbour, Wolfes Island, Taylor Head Provincial Park, Moosehead Cove, and Goldboro. Each sample site includes an eelgrass meadow (Zostera marina) in a shallow bay.","sampling":"Each site includes an eelgrass meadow for biodiversity monitoring. At each of the sites, we first collect water samples by boat from over top of the eelgrass beds. Three samples of 3 litres of water are collected and filtered per sample site. Samples are filtered with a Smith-Root portable vacuum pump on to a 1.2 micron PES filter to capture eDNA. At each site, a field blank consisting of 1-2 litres of distilled water are also filtered to check for potential contamination. These filters are frozen at -80 degrees Celsius until processing by the lab. At each site, we also conduct two beach seine hauls by boat, to collect fish and invertebrates in the eelgrass meadows. All animals collected are processed in the field and held in aerated totes of seawater. Invertebrates are identified but not counted, and all fish are identified and counted. A subset of 50-100 individuals per fish species are measured (total length) to obtain size distributions for each species. \n\nIn the lab, eDNA samples are extracted using commercial kits, and libraries for Illumina sequencing are created using primers for each marker of choice (e.g., MiFish-U for 12S targeting fish and Leray primers for COI targeting general eukaryotic biodiversity). These libraries are sequenced on an Illumina NovaSeq or MiSeq platform. Raw eDNA reads are demultiplexed and processed using the QIIME2 pipeline, with denoising and sequence merging conducted by DADA2. Amplicon sequence variant tables are created, and representative DNA sequences are assigned taxonomy using nucleotide BLAST against public reference databases.","methodSteps":["First, sample sites are chosen. These consist of accessible coastal areas with eelgrass meadows to examine the biodiversity living in these habitats. \nTriplicate 3-litre water samples are collected at each site by boat, and filtered using a Smith-Root vacuum pump with a 1.2 micron polyethersulfone filter. At each site, a single field blank consisting of 1-2 litres of distilled water is also filtered to check for potential contamination in the samples. \neDNA filters are preserved at -80 Celsius until processing in the laboratory. eDNA is then extracted using commercial kits. \neDNA libraries consisting of barcoded samples and their specific marker primers are created and sequenced on an Illumina MiSeq or NovaSeq platform. \nRaw eDNA samples are demultiplexed and processed using the QIIME2 pipeline, where primers and adapters are trimmed and sequences are merged and denoised in DADA2. Amplicon sequence variants (ASV) tables are generated by DADA2 for each ASV per sample site, and taxonomy is assigned to each ASV using nucleotide BLAST against the NCBI eukaryotic nucleotide database. The taxonomy is then filtered for relevant taxa (i.e., marine fishes and invertebrates) and manually curated to remove spurious observations."]},"countryCoverage":[],"collections":[],"dataDescriptions":[],"dataLanguage":"eng","acknowledgements":"This work was funded by the Marine Conservation Targets program with the department of Fisheries and Oceans Canada. ","pubDate":"2026-02-19T00:00:00.000+00:00","maintenanceUpdateFrequency":"ANNUALLY","maintenanceDescription":"Samples are collected from these coastal sites annually. Data will be uploaded to this resource each year following data processing.","license":"http://creativecommons.org/publicdomain/zero/1.0/legalcode","dwca":{"coreType":"http://rs.tdwg.org/dwc/terms/Occurrence","extensions":["http://rs.iobis.org/obis/terms/ExtendedMeasurementOrFact","http://rs.gbif.org/terms/1.0/DNADerivedData"],"modified":"2026-07-13T09:51:35.250+00:00"},"category":["eDNA"],"approximateCounts":{"occurrenceCount":12900}}