10.15468/spzi1j
https://ipt.biodiversity.aq/resource?r=soil_bacteria_king_george_island_and_deception_island
fca3f5da-a6ae-464d-a488-38439546056d
http://ipt.biodiversity.aq/resource?r=soil_bacteria_king_george_island_and_deception_island
V3-V4 16S rDNA of soil bacteria in King George Island and Deception Island
C.
Chua
Universiti Malaysia Sabah
Kota Kinabalu
88400
MALAYSIA
S.
Yong
Universiti Malaysia Sabah
Kota Kinabalu
88400
MALAYSIA
M.
Gonzalez
Instituto Antártico Chileno
Punta Arenas
CHILE
P.
Lavin
Instituto Antártico Chileno
Punta Arenas
CHILE
Y.
Cheah
Universiti Putra Malaysia
Serdang
43400
MALAYSIA
G.
Tan
University of Malaya
Kuala Lumpur
88400
MALAYSIA
C.
Wong
Universiti Malaysia Sabah
Kota Kinabalu
88400
MALAYSIA
Maxime
Sweetlove
Royal Belgian Institute for Naturals Sciences
Research assistent
Rue Vautier 29
Brussels
1000
msweetlove@naturalsciences.be
2019-03-19
ENGLISH
Amplicon sequencing dataset (Illumina MiSeq 250bp PE) of soil bacteria (16S ssu rRNA gene, v3-v4) on King George Island and Deception Island (Antarctica)
Metadata
GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml
This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
Creative Commons Attribution 4.0 International
https://spdx.org/licenses/CC-BY-4.0.html
CC-BY-4.0
King George Island and Deception Island, Antarctica
-60.68
-58.94
-62.19
-62.99
Bacteria, 16S ssu rRNA gene, v3-v4 region
Domain
Bacteria
Bacteria
unkown
C.
Chua
Universiti Malaysia Sabah
Kota Kinabalu
88400
MALAYSIA
C.
Wong
Universiti Malaysia Sabah
Kota Kinabalu
88400
MALAYSIA
Total bacterial genomic DNA was extracted from soils using the method described in Zhou et al. (1996) DNA recovery from soils of diverse composition. Appl. Environ. Microbiol., 62(2), 316–322.
The concentration and purity of the extracted genomic DNA were measured using the NanoDropTM 2000 spectrophotometer (Thermo Scientific Inc., Waltham, MA, USA).
The V3–V4 region of the bacterial 16S rRNA gene was amplified using locus-specific primers, S-DBact-0341-b- S-17 (5′-TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG CCT ACG GGN GGC WGC AG-3′)and S-D-Bact-0785-a-A-21 (5′-GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GGA CTA CHV GGG TAT CTA ATC C-3′). The 16S rDNA amplicons were subjected to paired-end sequencing on Illumina MiSeq platform using 2 × 250 bp MiSeq Reagent Kit v2 (Illumina® Inc., San Diego, California).
Soil samples were collected from King George Island (S62°11′35.3′′; W58°56′6.2′′) and Deception Island (S62°59′023′′; W60°40′51.7′′), Antarctica in February 2007.
Samples were stored at –20°C immediately after sampling.
V3-V4 16S rDNA of soil bacteria in King George Island and Deception Island
C.
Chua
ADMINISTRATIVE_POINT_OF_CONTACT
his work was supported by the Ministry of Science, Technology and Innovation (MOSTI), Malaysia under the Flagship project FP0712E012.
2026-04-10T09:54:02Z
Chua C, Yong S, Gonzalez M, Lavin P, Cheah Y, Tan G, Wong C, Sweetlove M (2019). V3-V4 16S rDNA of soil bacteria in King George Island and Deception Island. Version 1.1. SCAR - Microbial Antarctic Resource System. Metadata dataset https://doi.org/10.15468/spzi1j accessed via GBIF.org on 2026-04-10.
Chua, C. Y., Yong, S. T., González, M. A., Lavin, P., Cheah, Y. K., Tan, G. Y. A., & Wong, C. M. V. L. (2018). Analysis of bacterial communities of King George and Deception Islands, Antarctica using high-throughput sequencing. CURRENT SCIENCE, 115(9), 1701.