10.15468/7rhn8v https://ipt.ibbr.cnr.it/ipt/resource?r=cnr-ibe-cryobank-01 CNR-IBE-CRYOBANK-01 Carla Benelli CNR-IBE-FI, Institute of BioEconomy (IBE) - Section of Sesto Fiorentino (Florence), National Research Council of Italy Responsible of the IBE Cryobank

v. Madonna del Piano 10 Sesto Fiorentino FI IT-50019 ITALY

carla.benelli@ibe.cnr.it https://www.ibe.cnr.it/staff/?lettera=B 0000-0001-6068-8394 Maurizio Lambardi CNR-IBE-FI, Institute of BioEconomy (IBE) - Section of Sesto Fiorentino (Florence), National Research Council of Italy Responsible of the CryoCollections in liquid nitrogen of CNR/IBE

v. Madonna del Piano 10 Sesto Fiorentino FI IT-50019 ITALY

maurizio.lambardi@ibe.cnr.it 0000-0002-8898-7123 Anna De Carlo CNR-IBE-FI, Institute of BioEconomy (IBE) - Section of Sesto Fiorentino (Florence), National Research Council of Italy Responsible of the in vitro culture laboratory of IBE-CNR

v. Madonna del Piano 10 Sesto Fiorentino FI IT-50019 ITALY

anna.decarlo@ibe.cnr.it http://www2.ivalsa.cnr.it/2000/02/21/anna-de-carlo/ 0000-0002-8969-7945 Gabriele Bucci CNR-IBBR, Institute of Biosciences and BioResources, National Research Council of Italy Senior Researcher

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gabriele.bucci@cnr.it https://ibbr.cnr.it/info/people/gabriele-bucci 0000-0001-8931-0344 Dept. of Bio-Agrifood Sciences CNR-DiSBA National Research Council of Italy (CNR) Research Department

p.le Aldo Moro 7 Rome RM IT-00100 ITALY

CONTENT_PROVIDER Institute of Bioeconomy CNR-IBE National Research Council of Italy

Sesto Fiorentino Firenze IT-50019 ITALY

CONTENT_PROVIDER 2024-11-22 ENGLISH The Cryobank of IBE preserves in liquid nitrogen (-196 °C), the following accessions: (i) polyembrionic seeds from 24 accessions of Citrus spp. from the Medicean Collection of Villa Reale di Castello, in Florence (XVI° Century); (ii) dormant buds from 19 accessions of ancient varieties from the Veneto region, Italy; (ii) dormant buds from 3 accessions of plum (Prunus domestica) from the C.A.V.-Center of Nursery Activities of Tebano, Ravenna. Occurrence GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml micropropagation Citrus; Prunus domestica; Malus domestica; Tissue culture cryopreservation none Specimen GBIF Dataset Subtype Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_subtype.xml The collection has participated in the projects BioGenRes (http://www.biogenres.cnr.it/) and RGV-FAO 2020-2022 (http://www.politicheagricole.it/flex/cm/pages/ServeBLOB.php/L/IT/IDPagina/6760), in collaboration with C.A.V. of Tebano, Ravenna, for the duplication of apple germplasm. This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License. Creative Commons Attribution Non Commercial 4.0 International https://spdx.org/licenses/CC-BY-NC-4.0.html CC-BY-NC-4.0 https://biomemory.cnr.it/collections/CNR-IBE-CRYOBANK https://ipt.ibbr.cnr.it/ipt/archive.do?r=cnr-ibe-cryobank-01 Northern Italy, Central Italy, Tuscany, Emilia Romagna 11.22825 12.36749 44.91809 43.81941 2009-02-13 2015-03-31 Accessions of ancient varieties of Malus domestica, Prunus domestica, and Citrus spp. Family Rutaceae Order Rosales Class Magnoliopsida Family Rosaceae Kingdom Plantae Order Sapindales Phylum Tracheophyta Tissue culture, micropropagation, cryopreservation, liquid nitrogen. PNRR Project IR0000032—ITINERIS, Italian Integrated Environmental Research Infrastructures System—CUP B53C22002150006 (D.D. n. 130/2022) Funded by the EU—Next Generation EU—Mission 4 “Education and Research”—Component 2: “From research to business”—Investment 3.1: “Fund for the realization of an integrated system of research and innovation infrastructures”CNR-DiSBA Project No. SAC.AD002.173 BioMemory - Network of scientific collections for bio-monitoring, biodiversity conservation, agri-food and environmental sustainability, and well-being. The procedures utilized for cryopreservation are the desiccation and direct immersion in liquid nitrogen of polyembryonic seeds (Citrus) and the dormant bud technique for conserving uni-nodal segments of Malus domestica and Prunus domestica. asNeeded Carla Benelli CNR-IBE-FI, Institute of BioEconomy (IBE) - Section of Sesto Fiorentino (Florence) Reference Person

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carla.benelli@ibe.cnr.it https://www.ibe.cnr.it/staff/?lettera=B 0000-0001-6068-8394 Carla Benelli 0000-0001-6068-8394 POINT_OF_CONTACT Maurizio Lambardi 0000-0002-8898-7123 ORIGINATOR Anna De Carlo 0000-0002-8969-7945 AUTHOR Gabriele Bucci 0000-0001-8931-0344 PUBLISHER ITINERIS (PNRR Project IR0000032) Activity 6.5 "Mining and mapping the functional biodiversity in vivo and ex-situ research collections" - This activity aims to provide biodiversity (meta)data linked to Natural Science Collections (NSC) in terms of extended digital specimens fully linked to related information (e.g.. images and videos, codification of functional traits, DNA and protein sequences, environmental parameters, etc.). The expected outcome of the activity is the implementation of a national network of biological research collections, including all the existing in vivo and ex-situ collections of biological organisms (animals, plants, fungi, algae, archaea, bacteria, viruses, etc.) that have been created and maintained through past and current research projects. || BIOMEMORY (CNR-DiSBA Project No. SAC.AD002.173) is the network of biological collections of the Department of Biology, Agriculture and Food Sciences (DiSBA) of the National Research Council of Italy (CNR) for bio-monitoring, biodiversity conservation, agri-food and environmental sustainability, and human well-being. The project aims to create a network of biobanks (i.e., scientific research collections) where data and metadata associated with biological samples of different natures are collected and stored systematically and well-organized. Maintaining the existing collections will allow their future use for many purposes, from the genetic improvement of organisms to face environmental changes (climate-ready organisms) to the fight against epidemics and pandemics affecting humans, animals, and plants. ITINERIS project no. IR0000032 — ITINERIS, Italian Integrated Environmental Research Infrastructures System—CUP B53C22002150006 (D.D. n. 130/2022) Funded by the EU—Next Generation EU—Mission 4 “Education and Research”—Component 2: “From research to business”—Investment 3.1: “Fund for the realisation of an integrated system of research and innovation infrastructures” || BIOMEMORY (CNR-DiSBA Project No. SAC.AD002.173 Consiglio Nazionale delle Ricerche https://doi.org/10.13039/501100004462 CNR-DiSBA no. SAC.AD002.173 https://www.disba.cnr.it/en/ Ministero dell'Università e della Ricerca https://doi.org/10.13039/501100004462 PNRR Project IR0000032 https://itineris.cnr.it/ 2026-04-16T10:02:41Z Benelli C, Lambardi M, De Carlo A, Bucci G (2024). Cryobank of Tissues from Ancient Varieties of Fruit Trees (CNR-IBE-CRYOBANK-01). Consiglio Nazionale delle Ricerche, Istituti del Dipartimento di Scienze Bio-AgroAlimentari (CNR-DiSBA). Occurrence dataset https://doi.org/10.15468/7rhn8v accessed via GBIF.org on 2026-04-16. API-JSON UTF-8 Darwin Core https://biomemory.cnr.it/api/occurrences/json/?dataset=134 https://www.cnr.it/it/istituto/131/logo 78c9b49e-d4a3-4451-b3b3-ef34cf06caa4 Cryobank of Fruit Trees Tissues DRIED