Colección de bacterias aeróbicas formadoras de endospora aisladas de la rizosfera y filosfera de plantas de banano y plátano del Urabá Antioqueño.
\nEl presente conjunto de datos cuenta con 1.493 registros biológicos (RR.BB) colectados en el departamento de Antioquia, desde el año 2009 al 2019. Los RR.BB pertenecen al reino Bacteria, filo Bacillota, clase Bacilli, orden Caryophanales. Un 7.3% está clasificado a nivel de especie, un 3.6% a género, 3.01% a familia y un 86% a orden.
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conjunto de datos cuenta con 1.493 registros biológicos (RR.BB). Las bacterias aisladas de plantas de banano y plátano, son todas bacterias aeróbicas formadoras de endospora del orden Caryophanales. El 7.3% está clasificado a nivel de especie, un 3.6% a género, 3.01% a familia y un 86% a orden. Algunas especies representativas de la colección son: Bacillus velezensis, Bacillus subtilis; Bacillus amyloliquefaciens, Bacillus pumilu, Bacillus tequilensiss, Bacillus xiamenensis, Bacillus amyloliquefaciens.","coverages":[{"scientificName":"Bacillaceae","rank":{"verbatim":"family","interpreted":"FAMILY"}},{"scientificName":"Paenibacillaceae","rank":{"verbatim":"family","interpreted":"FAMILY"}}]}],"geographicCoverages":[{"description":"Bacterias aisladas de la filosfera y rizosfera de plantas de banano ubicadas en tres fincas bananeras del Urabá Antioqueño (Colombia): Campo experimental de Augura (7.779444, -76.666722), Finca la Navarra (7.866111, -76.6275), Finca el Aserrío (7.873611, -76.633406)","boundingBox":{"minLatitude":7.779,"maxLatitude":7.874,"minLongitude":-76.668,"maxLongitude":-76.628,"globalCoverage":false}}],"temporalCoverages":[{"@type":"range","start":"2009-10-19T00:00:00.000+00:00","end":"2019-11-30T00:00:00.000+00:00"}],"keywordCollections":[{"thesaurus":"n/a","keywords":["Paenibacillus","Bacillales","Bacillus","bacterias"]},{"thesaurus":"GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml","keywords":["Occurrence"]},{"thesaurus":"GBIF Dataset Subtype Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_subtype.xml","keywords":["Specimen"]}],"project":{"title":"DISEÑO DEL PROCESO PRODUCTIVO Y DESARROLLO DE UN PRODUCTO CON BASE EN BACTERIAS DEL FILOPLANO DEL GÉNERO Bacillus PARA EL CONTROL DE SIGATOKA NEGRA EN BANANO Musa AAA.","contacts":[{"type":"PRINCIPAL_INVESTIGATOR","primary":false,"userId":[],"firstName":"Valeska","lastName":"Villegas Escobar","position":[],"email":[],"phone":[],"homepage":[],"address":[]}],"funding":"Universidad EAFIT, AUGURA y Colciencias","awards":[],"studyAreaDescription":"La investigación se llevó a cabo en la Unviersidad EAFIT (Medellín, Colombia) y en Cenibanano - Augura (Carepa, Antioquia).","relatedProjects":[]},"samplingDescription":{"studyExtent":"Bacterias aisladas de la filosfera y rizosfera de plantas de banano ubicadas en tres fincas bananeras del Urabá Antioqueño (Colombia): Campo experimental de Augura (7.779444, -76.666722), Finca la Navarra (7.866111, -76.6275), Finca el Aserrío (7.873611, -76.633406).","sampling":"Las muestras de raíces y hojas de plantas de banano fueron colectadas de las diferentes fincas bananeras y enviadas a Medellín. Las muestras fueron procesadas antes de 48h de recolectadas las muestras. Las bacterias aeróbicas formadoras de endospora fueron aisladas de los tejidos mediante siembra en medios de cultivo sólidos para el recuento de unidades formadoras de colonia (UFC/g de tejido). Diferentes morfotipos de bacterias presentes en los aislamientos fueron seleccionados, purificados y conservados a -80°C.","methodSteps":["Las Muestras de hojas de banano se obtuvieron del campo experimental AUGURA. En cada plantación se seleccionaron 5 puntos para recolectar muestras compuestas de tres plantas antes de la floración mediante un muestreo probabilístico sin reposición. Se tomaron muestras de la posición de las hojas 2, 5 y 10 de cada planta, y cada hoja se dividió en cuatro partes iguales. Luego, se seleccionó para el análisis una parte del ápice y otra de la base.","El aislamiento de bacterias epífitas se realizó con modificaciones a la metodología propuesta por un Yadav et al. 2005, lavando las muestras en un tampón de fosfato de sodio estéril. Las muestras se diluyeron en serie y 100 μL de cada dilución se sembraron en Agar Tripticase Soya (TSA). La población total de bacterias epífitas se estimó después de 48 h de incubación a 30 °C para aquellas diluciones que contenían 50–100 CFU por placa. Para el conteo total de bacterias epífitas y endófitas, las muestras sonicadas se homogeneizaron en una licuadora durante 10 s y, posteriormente, se realizó el mismo procedimiento de diluciones seriadas y placas. El conteo de bacterias cultivables en la filosfera se realizó para muestras de diferentes zonas foliares, posición de las hojas y plantas de diferentes cultivares de banano y plátano de Urabá (Colombia) con el fin de identificar la influencia de estas fuentes en la variabilidad (zona foliar, posición y cultivar) del tamaño de la población de bacterias epífitas y endófitas.","Para seleccionar bacterias formadoras de endosporas (AEFB), se determinó la clasificación de Gram de cada colonia de acuerdo con el protocolo de Buck. Las bacterias grampositivas se purificaron en TSA al 10 % y se cultivaron en medios de Finley y Field. Los caldos de cultivo resultantes se sometieron a choque térmico y se sembraron en TSA para seleccionar bacterias. Todos los AEFB se almacenaron a -80 °C en Tripticase Soya Broth (TSB) con glicerol.","Las muestras de raíces se recolectaron de las raíces de tres Musa spp. cultivares (plátano cv. Grand Naine y banano cv. Valery que pertenece al grupo de genoma AAA y subgrupo Cavendish, y plátano cv. Harton que pertenece al grupo de genoma AAB) en Urabá, Colombia (región nororiental).\nPara plátano cv. Grand Naine, se obtuvieron muestras de raíces de las plantaciones 1, 2 y 3; para cv. Valery, de las plantaciones 1 y 4; y para plátano cv. Harton, de las plantaciones 1 y 5. En cada plantación, se recolectaron muestras compuestas de raíces de tres plantas adyacentes (en etapa de prefloración) a 5–10 cm de profundidad se almacenaron en bolsas de propileno a 4 ◦C y se procesaron en 24 h.","El aislamiento de AEFB se llevó a cabo suspendiendo 40 g de raíces con suelo de rizosfera adherido en 400 ml de tampón de fosfato de sodio, sonicando la suspensión durante 10 min. en un limpiador de ultrasonidos. A continuación, las suspensiones se colocaron en un agitador orbital durante 1 h y se homogeneizaron en una licuadora durante 15 s. Las suspensiones se diluyeron en serie y se sometieron a un tratamiento térmico y luego, 100 l de cada dilución se sembraron en TSA. Se seleccionaron, purificaron y almacenaron diferentes morfotipos a -80 ◦C en TSB con glicerol."]},"countryCoverage":[],"collections":[{"name":"Colección de bacterias aisladas de Musa sp.","identifier":"Registro Nacional de Colecciones Biológicas:191","parentIdentifier":"EA-CB","specimenPreservationMethod":"DEEP_FROZEN","curatorialUnits":[]},{"name":"Colección de bacterias aisladas de Musa sp.","identifier":"https://scientific-collections.gbif.org/collection/361186de-cb0f-4ab8-9d94-d168e5411df3","parentIdentifier":"EA-CB","curatorialUnits":[]}],"dataDescriptions":[],"dataLanguage":"spa","additionalInfo":"[autoridad]CORPOURABA[numero]200-03-20-01-1312-2009[titular]Universidad EAFIT[nit o cedula]890901389-5[fecha]2009-10-19","pubDate":"2024-09-10T00:00:00.000+00:00","maintenanceUpdateFrequency":"UNKOWN","maintenanceDescription":"","license":"http://creativecommons.org/licenses/by-nc/4.0/legalcode","dwca":{"coreType":"http://rs.tdwg.org/dwc/terms/Occurrence","extensions":["http://data.ggbn.org/schemas/ggbn/terms/Permit"],"modified":"2026-03-25T03:45:05.533+00:00"}},{"key":"c9e31c10-6986-4795-8244-30ad2486d0bb","installationKey":"c2f1d0d3-c5dc-4995-835d-d04e4ec46d6e","publishingOrganizationKey":"05c249d0-dfa0-11d8-b22e-b8a03c50a862","doi":"10.15468/2zv874","version":"1.6","external":false,"numConstituents":0,"type":"OCCURRENCE","subtype":"SPECIMEN","title":"Florient - a database dealing with historic wild plant occurrences","description":"Florient - a database dealing with historic wild plant occurrences is an occurrence dataset published by Ghent University and the Belgian Biodiversity Platform.\nThe FLORIENT database is based on the herbarium of Charles Van Hoorebeke (1790-1821), in which he recorded nearly all wild plants that occurred in the province of East Flanders. A prospectus of his planned publication about this, the \"Flore de la Flandre Orientale\", appeared in 1821. That same year he died. As a result of which his work never appeared in print and the data were not available. Frans Roucel (1765-1831), a contemporary from Aalst, focused in his published floras (from 1792 and 1803) on rare wild plants and their use, and went into more detail about the growing conditions of the species. The combination of information from both works provides an exceptionally complete and detailed picture of the flora of East Flanders around the beginning of the 19th century, before the industrial and agricultural revolution.\nThe digital collection is also available at www.botanicalcollections.be
\nThis dataset contains historic wild plant occurrences (early 19th century). Here it is published as a standardized Darwin Core Archive and includes for each record an eventID, date, location, sampling protocol, occurrenceID, the number of recorded individuals, status (present/absent), . Issues with the dataset can be reported at https://github.com/BelgianBiodiversityPlatform/data-publication-bbpf/tree/main/datasets/florient-occurrences
\nGeneralized and/or withheld information: as these are historic occurrences, the location is geocoded to the municipality as it originally appeared in the data.
\nWe have released this dataset to the public domain under a Creative Commons Zero waiver. We would appreciate it if you follow the INBO norms for data use (https://www.inbo.be/en/norms-data-use) when using the data. If you have any questions regarding this dataset, don't hesitate to contact us via the contact information provided in the metadata.
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\nEsta colección esta conformada por dos grandes grupos de microorganismos. El primer grupo, corresponde a bacterias aisladas del tracto gastrointestinal de herbívoros silvestres y el segundo, por bacterias ácido-lácticas provenientes de ensilajes. Por sus características el primer grupo son microorganismos anaerobios estrictos, en su mayoría con potencial celulítico. El grupo de bacterias Ácidolácticas son bacterias Gram positivas, catalasa negativo y en su mayoría con potencial biocontrolador y probiótico.
\nEste recurso contiene la información de 191 microorganismos actualmente presentes en la colección y de 145 que han sido dados de baja, estos datos han sido tomados entre 1996 al 2022 en 10 departamentos de Colombia.
\nAdemas contiene información de la actividad antagónica contra algunas bacterias en el campo dynamicProperties. Adicionalmente, en las extensiones contiene información del medio de cultivo, métodos de preservación y datos de primers para la amplificación.
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